LOCUS       ISCR1-qnrB2_unit        4659 bp    DNA     linear   BCT 26-OCT-2020
DEFINITION  Klebsiella pneumoniae.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella
            pneumoniae.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR1-qnrB2_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
     mobile_element  1..4659
                     /gene="ISCR1-qnrB2 unit"
                     /product="Putative resistance unit: ISCR1-qnrB2 unit"
     mobile_element  complement(1..2154)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             381..1922
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     CDS             complement(2044..2694)
                     /gene="sapA"
                     /codon_start=1
                     /transl_table=11
                     /product="Peptide transport periplasmic protein SapA"
                     /translation="MRLVLSSLIVMAGFLSGQAAAAPEQAPHADIRDSGFVYCVSGQV
                     NTFNPQKTSSGLIVDTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYRFHLRR
                     DVQFQKTAWFTPTRKLNADDVVFTFERIFDRHHPWHNVNGSSFPYFDSLQFADNVQSV
                     RKLDNNTVEFRLTQPDASFMGYRKYKPPFCSSSEVSYLKFPHSFTAQAPILTAGPA"
     misc_recomb     2134..2154
                     /gene="oriIS"
                     /product="oriIS of ISCR1"
     CDS             3220..3681
                     /gene="orf462"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MNCMNKLLSLVVSLALLPGAVFAASTPDCVKVNKAQIEALFDKW
                     NESLKTGNAQTVSENYLSDAVLLPTVSNKARLTDAERVDYFEHFLAKKPTGKIDMRTI
                     RLGCNKAIDTGTYTFTFADKSTVSARYTFTYAWDGKEWKISTHHSSAMPEG"
     CDS             complement(3723..4367)
                     /gene="qnrB2"
                     /codon_start=1
                     /transl_table=11
                     /product="Quinolone resistance protein"
                     /translation="MALALVGEKINRNRFTGEKIENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNASALGIEIRHCRAQGADFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGAQVLGATFSGSDLSGGE
                     FSTFDWRAANFTHCDLTNSELGDLDIRRVDLQGVKLDNYQASLLMERLGIAIIG"
BASE COUNT     1207 a   1173 c   1126 g   1153 t
ORIGIN
        1 aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa
       61 agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag
      121 ctcgacgact accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat
      181 aacagttggg gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt
      241 ctcgctcaac cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta
      301 gcgatgtcta gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac
      361 agtatcagcg gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca
      421 ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg
      481 agcagcacta cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct
      541 acatccaaca agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc
      601 gggttcgctg cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg
      661 gcttttgccc tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg
      721 aagtcttccc caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct
      781 ttttgctggc tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac
      841 tctcaactca tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag
      901 tgactcttat ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt
      961 ttctcgatgg tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg
     1021 cacccactta cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct
     1081 gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag
     1141 agggcgaaga cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg
     1201 tcggccccca gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata
     1261 aagccgactc aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga
     1321 tggccgaagc gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag
     1381 cggtttcaga aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa
     1441 ctccgtaccg caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac
     1501 tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac
     1561 cgaacagcaa acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat
     1621 cggaaggtct cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga
     1681 cctggatgca acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg
     1741 gcggtcacgt caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca
     1801 agcatctgaa acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag
     1861 aacgagcgcc gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact
     1921 gacgacccca aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag
     1981 tttgtatctc tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc
     2041 ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt
     2101 caggtaagat acttcggatg aggagcaaaa aggtggttta tacttcctat accccataaa
     2161 ggaggcatcc ggttgcgtta agcggaattc aacggtgttg ttgtccagtt tgcgcacgct
     2221 ttggacgtta tcggcaaact gcaggctgtc gaagtagggg aaactgctgc cgttcacatt
     2281 gtgccacgga tggtggcgat caaagatgcg ctcgaaggta aacaccacat cgtctgcatt
     2341 cagtttgcgg gtgggggtga accaggccgt cttctgaaac tgaacgtcac gacgcagatg
     2401 gaagcggtag gttgcgccgt tatccagcac ctcccagctt tcagcaagct caggcaccag
     2461 ccgataggtg tagggatcta catcaagcag tcggtcatac aattgggccg ctaacgtatc
     2521 gacgatgaga ccgctgctcg ttttttgtgg gttgaacgta ttgacttgcc cgctgacgca
     2581 atagacaaac ccactgtcac ggatatcagc gtgcggagct tgctcaggcg cagcagcagc
     2641 ctgaccactc agaaatccag ccatcacgat cagagatgat aaaaccaggc gcataatttt
     2701 aatggattat ataaagaggc tatcttacta atacttaatg acatttgcca ttaccgtttg
     2761 tttttgggag taacaggctc gataagcgcg agttcaacac cgcgacatgg tcaaaattca
     2821 tacctgctat ccactttgaa tctactctgt taactatctt agcattttca tggcccatct
     2881 gacttgctat aaacgtgggg gcgtacctgt cgttgtttac tgattgatat cagtgtgtgc
     2941 tcggaagatg gagtatggca tcgataacga agtgtggagc gagcatttag agtgtcacta
     3001 tcaatgctga actataatca ttcaggaagc agtacacgtt atgtgagttg taatagctaa
     3061 agtatctata attatttcaa tatgttaatt ccattttttc ttcatggcat cttaatcttc
     3121 tcacccattt atggtgatga tttccattgg ttattaaatg cattttggct ttgatcctgg
     3181 ttatgaacgg ttttctgttg ccatttaact tctaatggaa tgaattgtat gaataaatta
     3241 ctgagccttg ttgtttcact tgccctattg cctggagccg tttttgcagc gtcgactcca
     3301 gactgtgtga aggtgaataa agcgcaaatc gaagcgctat ttgataagtg gaacgagtca
     3361 ttgaaaacag gtaacgcgca gacggtatct gaaaattatc ttagcgatgc ggtattgttg
     3421 cctactgtat ccaataaagc gcgtttaacg gacgctgaac gtgttgatta tttcgagcat
     3481 tttcttgcga aaaagccgac cgggaaaatt gacatgcgta ccattcgcct ggggtgtaat
     3541 aaggcgatag acaccggtac ctacacattt accttcgcgg acaaatcaac ggtttcagcc
     3601 cgctacacat tcacttatgc atgggacggt aaagagtgga aaatttccac acaccactct
     3661 tcagcgatgc ctgaagggta aaaagctgtg cagggggcgg aatattcacc gctccctgaa
     3721 gactagccaa taatcgcgat gccaagacgt tccatgagca acgatgcctg gtagttgtcc
     3781 aacttaacgc cttgtaaatc aacgcgccga atatctaagt cacccaactc cgaattggtc
     3841 agatcgcaat gtgtgaagtt tgctgctcgc cagtcgaaag tcgaaaactc gccgccggag
     3901 agatctgaac cactgaacgt cgcgcccagt acctgggccc ccatccaacg gttttcccac
     3961 agctcacact tttccaacac gactttcgaa aaattggcgt agcttagatt tgtgttagtg
     4021 atatatgcac tgcaaaacca ggtgcgagta gtgatcatat tcataaaact tgcgccgcga
     4081 aaatctgcgc cttgcgcacg acagtggcga atttcaatgc caagcgcact ggcattgcga
     4141 aaatccgcca tggataaatc acagctttta aaaatggcat ctttcagcat cgcacgacta
     4201 aaattgcacc ctttctggct ttcacgatca tagaactgac agccgataaa ttcagtgccg
     4261 ctcaggtcgg cacctgaaaa atcacagtta aaaaatgtac tattttcaat tttctcaccg
     4321 gtgaagcggt ttctgttaat tttttcgcca acgagtgcca gagccatatt ttgtgcctgt
     4381 ttttttatac agtaatggcg tcatggtaaa ccctgatgag gttatgcgtc aaatccgcca
     4441 atataacatc tgcaaatgtg cgttaaatct ggtgtttttt cagcaaagcg cgaagctgat
     4501 ggtaagtcag acccagtaat tcagcggctt ttttctggtt aaattttgcc tgctgtaagc
     4561 tggtttgtag aaagtctttc tcttgctgct gctggaattc acgcagatcc agcggtaacc
     4621 ctacagacat ctgtttagtt tccggcgcct gcggctgcg
//
